Links to the WWW qPCR Newsgroups
The Web Guide of Polymerase Chain Reaction Technique. for further information please contact webmaster@pcrlinks.com
http://www.cyclertest.com/
CYCLERtest is an independent company dedicated to perform and to develop the most sophisticated and accurate multichannel temperature validation systems, tests and techniques available on the market at this point in time, all of which must be compliant with and related to reliable industry standards.
PCR and RT-PCR
PCR and RPA & others movies under
by Dolan DNA Learning Center, Cold Spring Habor Laboratory
www.highveld.com/pcr.html
The ultimate Web page for information and links on all aspects of the Polymerase Chain Reaction The polymerase chain reaction (PCR) is used in a wide variety of fields including: molecular biology, environmental science, forensic science, medical science, biotechnology, microbiology, the food industry, diagnostic science, epidemiology, genetics, gene cloning, and many more. This site provides scientists with a comprehensive directory for the polymerase chain reaction (PCR) and related techniques.
The Lundberg laboratory was among the pioneers in quantitative real-time PCR and contributed by inventing the light-up probes. We now strengthen our capability to handle external relations and take on commercial projects within the field of Q-PCR.
Amongst TATAA Biocenter activities:
Designed by OCTAVIAN HENEGARIU, Yale University
http://info.med.yale.edu/genetics/ward/tavi/PCR.html
Quantitative Real-Time Polymerase Chain Reaction for the Core Facility Using TaqMan and the Perkin-Elmer/Applied Biosystems Division 7700 Sequence Detector
by Deborah S. Grove Nucleic Acid Facility, Life Science Consortium, The Pennsylvania State University, University Park, PA 16802
TechNotes 8 (1): real-time PCR Goes Prime Time
Real-time PCR assays used for quantitative RT-PCR combine the best attributes of both relative and competitive (end-point) RT-PCR in that they are accurate, precise, capable of high throughput, and relatively easy to perform. To truly appreciate the benefits of this technology, a review of PCR fundamentals is necessary.
http://www.labvelocity.com/protocols/index.jhtml
PCR and multiplex PCR: guide and troubleshooting http://info.med.yale.edu/genetics/ward/tavi/PCR.html
PCR Applications Manual. Roche Molecular Biochemicals-Research http://biochem.boehringer-mannheim.com/prod_inf/manuals/pcr_man/pcr_toc.html
Alkami Biosystems Primer Design Tips http://www.alkami.com/primers/refprmr.htm
BioGuide - PCR http://bioinformatics.weizmann.ac.il/mb/bioguide/pcr/contents.html
PCR-based sequence tagged sites have been used as landmarks for construction of various types of genomic maps. Using NCBI's "electronic PCR" program, these sites can be detected in DNA sequences, potentially allowing their map locations to be determined. http://www.ncbi.nlm.nih.gov/STS/
Polymerase Chain Reaction (PCR) Jump Station - An excellent web page for links and information on PCR. http://www.horizonpress.com/pcr/
Amplification Assistant PCR Troubleshooting Program. http://www.promega.com/amplification/assistant/
DNA and PCR methods http://www.nwfsc.noaa.gov/protocols/methods/DNA-PCRMethodsMenu.html
Applied Biosystems Sequence Detection Systems - NEWS
ABI-PRISM 7700 FAQs & Tutorials & Technical Notes & Multimedia Presentations
Products for LightCycler
Transcript of a Webcast on Real-Time PCR Applications (Bio.Com)
An excellent review on Quantitative Real-Time PCR (Bustin SA, 2000)
A review on Product Differentiation by DNA Melting Point Curves (Ririe et al, 1997)
QiaGen Handbooks on SYBR Green Detection Systems and RT-PCR and SYBR Green Kit
A Biocompare article on real-Time PCR platforms
A Review of Molecular Beacons (by Robinson JK et al. )
A technical article on Fluorescence Excitation and Emission on IDT Website
The COMPLETE Alkami Quick GuideTM for PCR is a 158 page manual with a file size of 296 KB. It is formated to be 5.5" X 8.5" in order to make the Guide a convenient size for use at your bench. The complete Guide will print out on 79 single-sided, letter sized, 8.5" X 11" sheets which you can cut and bind as desired. Click [HERE] to download your FREE copy of the Adobe Acrobat Reader.
PCR Primer design PCR Methods PCR Polymerases PCR Variables PCR Troubleshooting
Appendixes Comprehensive Index
by Carl Wittwer is Professor of Pathology at the University of Utah Medical School and Director of Flow Cytometry and New Technology at Associated Regional and University Pathologists (ARUP), and Chief Technology Officer at Idaho Technology.
Applications of Rapid Cycle Real Time PCR
This presentation requires the RealPlayer 8 Basic.
Quantification on the LightCycler® Instrument
Calculation of the efficiency, "fit point method" vs. "2nd derivate maximum method", noise-band, hybridisation probes, etc. by Randy Rasmussen Ph.D. (Idaho Technology, Chief Operating Officer) http://www.idahotec.com/lightcycler_u/lectures/quantification-on-lc.htm